Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin production by human embryonic stem cells.

S Assady1, G Maor, M Amit

  • 1Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, and. Rambam Medical Center, Bat-Galim, Haifa, Israel.

Diabetes
|July 27, 2001
PubMed
Summary

Human embryonic stem cells (hES cells) can spontaneously differentiate into insulin-producing beta-cells in vitro. This finding offers a potential new source for cell replacement therapy in type 1 diabetes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perineural Invasion Exhibits Traits of Neurodegeneration.

Journal of dental research·2025
Same author

Advances in Head and Neck Cancer Pain.

Journal of dental research·2022
Same author

Upregulation of RET induces perineurial invasion of pancreatic adenocarcinoma.

Oncogene·2017
Same author

Prognostic Performance of Current Stage III Oral Cancer Patients After Curative Intent Resection: Evidence to Support a Revision of the American Joint Committee on Cancer Staging System.

Annals of surgical oncology·2015
Same author

Elective neck dissection during salvage total laryngectomy--a beneficial prognostic effect in locally advanced recurrent tumours.

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2014
Same author

Maternal lipopolysaccharide alters the newborn oxidative stress and C-reactive protein levels in response to an inflammatory stress.

Journal of developmental origins of health and disease·2014

Area of Science:

  • Stem cell biology
  • Endocrinology
  • Diabetes research

Background:

  • Type 1 diabetes is caused by autoimmune destruction of pancreatic beta-cells, leading to insulin deficiency.
  • Current cell replacement therapies for diabetes are limited by donor availability.

Purpose of the Study:

  • To investigate the potential of human embryonic stem (hES) cells for generating insulin-producing beta-cells.
  • To explore hES cells as an alternative source for cell replacement therapy in diabetes.

Main Methods:

  • Used pluripotent undifferentiated hES cells for lineage-specific differentiation.
  • Cultured hES cells under both adherent and suspension conditions.
  • Analyzed cells using immunohistochemical staining for insulin and other beta-cell markers.

Related Experiment Videos

  • Measured insulin secretion into the culture medium.

    • Main Results:

    • Observed spontaneous in vitro differentiation of hES cells into cells with beta-cell characteristics.
    • Detected insulin expression in a high percentage of differentiated cells.
    • Confirmed differentiation-dependent insulin secretion.
    • Identified other beta-cell markers in the differentiated cells.

    Conclusions:

    • hES cells serve as a valid model system for generating insulin-producing cells.
    • These findings support the potential of hES cells as a future source for beta-cell replacement therapy in diabetes.