Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytosolic phospholipase A(2) activity during the ongoing cell cycle.

G S van Rossum1, A S Vlug, H van den Bosch

  • 1Department of Molecular Cell Biology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands.

Journal of Cellular Physiology
|July 27, 2001
PubMed
Summary

Cytosolic phospholipase A(2) (cPLA(2)) activity fluctuates during the cell cycle, peaking in mitosis and increasing at the G1/S transition. This regulation is driven by cPLA(2) phosphorylation via MAPK, not expression changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum to: Identification of a restriction point at the M/G1 transition in CHO cells.

Cellular and molecular life sciences : CMLS·2017
Same author

Immunohistochemical and serological investigation of experimental Ornithobacterium rhinotracheale infection in chickens.

Avian pathology : journal of the W.V.P.A·2016
Same author

[Genome composition analysis of the reassortant influenza viruses used in seasonal and pandemic live attenuated influenza vaccine].

Molekuliarnaia genetika, mikrobiologiia i virusologiia·2012
Same author

Three-dimensional quantification of regional left-ventricular dyssynchrony by magnetic resonance imaging.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2012
Same author

Skin lesions depicting a systemic disease. Weber-Christian disease.

The Netherlands journal of medicine·2011
Same author

Gridded Aclar: preparation methods and use for correlative light and electron microscopy of cell monolayers, by TEM and FIB-SEM.

Journal of microscopy·2010

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Cytosolic phospholipase A(2) (cPLA(2)) selectively releases arachidonic acid, a key mediator in cellular responses.
  • Arachidonic acid release and prostaglandin synthesis are linked to the cell cycle.
  • Understanding cPLA(2) regulation is crucial for cellular signaling research.

Purpose of the Study:

  • To investigate the cell cycle-dependent activity of cPLA(2).
  • To elucidate the regulatory mechanisms controlling cPLA(2) activity throughout the cell cycle.

Main Methods:

  • Cell synchronization using the mitotic shake-off method.
  • Measurement of cPLA(2) activity in synchronized neuroblastoma and Chinese hamster ovary cells.
  • Analysis of cPLA(2) expression and phosphorylation.

Related Experiment Videos

  • Utilizing MAPK kinase inhibitors and serum depletion to assess regulatory pathways.

    • Main Results:

    • cPLA(2) activity was highest in mitotic cells and decreased in early G1 phase.
    • A significant increase in cPLA(2) activity was observed at the G1/S transition.
    • Changes in cPLA(2) activity correlated with phosphorylation, not expression levels.
    • MAPK signaling was identified as the pathway responsible for cPLA(2) phosphorylation and activity modulation.

    Conclusions:

    • cPLA(2) activity is tightly regulated in a cell cycle-dependent manner.
    • Phosphorylation, mediated by MAPK, is the primary mechanism controlling cPLA(2) activity during the cell cycle.
    • These findings provide insights into the role of cPLA(2) in cell cycle progression and signaling.