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Related Experiment Videos

Identification of plant-associated enterococci.

T Müller1, A Ulrich, E M Ott

  • 1Centre for Agricultural Landscape and Land Use Research Müncheberg, Institute of Primary Production and Microbial Ecology, Müncheberg, Germany. tmueller@zalf.de

Journal of Applied Microbiology
|July 28, 2001
PubMed
Summary

This study identified novel Enterococcus species from forage grass using molecular methods. Most isolates showed resistance to gentamicin and streptomycin, suggesting a new plant-associated species.

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Area of Science:

  • Microbiology
  • Bacterial Taxonomy
  • Antimicrobial Resistance

Background:

  • Enterococcus species are increasingly recognized for their roles in various environments, including agricultural settings.
  • Understanding the diversity and characteristics of Enterococcus isolates from forage grass is crucial for ecological and potential health implications.

Purpose of the Study:

  • To taxonomically classify Enterococcus isolates obtained from forage grass.
  • To investigate the antibiotic resistance profiles of these Enterococcus isolates.

Main Methods:

  • Phenotypic characterization and molecular techniques including 16S ribosomal DNA (rDNA) restriction analysis, whole-cell protein profiling, and 16S rDNA sequencing were employed.
  • Antibiotic resistance was assessed using agar diffusion tests.

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Main Results:

  • The isolates were identified as Enterococcus faecium, Ent. mundtii, Ent. casseliflavus, Ent. faecalis, and Ent. sulfureus.
  • A distinct group of isolates, designated 16S rDNA genotype VI, showed unique restriction patterns and shared 97.4% 16S rDNA sequence similarity with Ent. faecalis.
  • All isolates were sensitive to vancomycin, but highly resistant to gentamicin and streptomycin.

Conclusions:

  • The taxonomical data strongly suggest that the 16S rDNA genotype VI isolates represent a novel species of Enterococcus associated with plants.
  • The observed antibiotic resistance patterns highlight potential challenges in managing these bacteria in agricultural contexts.