Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A molecular code for splicing silencing: configurations of guanosine-rich motifs.

Biochemical Society transactions·2004

Same author

Alternative pre-mRNA splicing and neuronal function.

Progress in molecular and subcellular biology·2002

Same author

A CaMK IV responsive RNA element mediates depolarization-induced alternative splicing of ion channels.

Nature·2001

Same author

Protein diversity from alternative splicing: a challenge for bioinformatics and post-genome biology.

Cell·2000

Same author

Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.

Molecular and cellular biology·2000

Same author

Multisite RNA binding and release of polypyrimidine tract binding protein during the regulation of c-src neural-specific splicing.

Molecular cell·2000

Search research articles

Related Experiment Video

Updated: Jul 19, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Alternative RNA splicing in the nervous system.

P J Grabowski¹, D L Black

¹Department of Biological Sciences, Howard Hughes Medical Institute, A507LH, University of Pittsburgh, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA. pag4@pitt.edu

Progress in Neurobiology

|July 28, 2001

Summary

Alternative splicing generates diverse proteins in the nervous system, crucial for brain function and development. Misregulation of this genetic process contributes to neurological diseases.

More Related Videos

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Related Experiment Videos

Last Updated: Jul 19, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular Biology
Neuroscience
Genetics

Background:

Alternative splicing is a key regulatory mechanism controlling gene expression.
It generates multiple messenger RNA (mRNA) isoforms from a single pre-mRNA.
This process is particularly prevalent and vital in the nervous system.

Purpose of the Study:

To review the biological phenomenon of alternative splicing in the nervous system.
To discuss its biochemical mechanisms and the role of RNA binding proteins.
To explore its implications in neurological diseases.

Main Methods:

Literature review of current research on alternative splicing.
Analysis of biochemical mechanisms underlying alternative splicing.
Discussion of tissue-specific properties of RNA binding proteins.

Main Results:

Alternative splicing generates significant proteomic diversity in the nervous system.
Specific mRNA isoforms play critical roles in neuronal functions like learning, memory, and neurotransmission.
Dysregulation of alternative splicing is linked to various human neurological diseases.

Conclusions:

Alternative splicing is essential for nervous system complexity and function.
Understanding its mechanisms is vital for addressing neurological disorders.
Further research is needed to fully elucidate its role and control in the brain.