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Related Experiment Videos

Lack of decrease in hypothalamic and hippocampal glucocorticoid receptor mRNA during starvation.

S Makino1, T Kaneda, M Nishiyama

  • 12nd Department of Internal Medicine, Kochi Medical School, Okoh-cho, Nankoku, Kochi, Japan. fwjf6671@mb.infoweb.ne.jp

Neuroendocrinology
|July 28, 2001
PubMed
Summary

Starvation maintains glucocorticoid receptor (GR) mRNA levels despite high corticosterone, preserving negative feedback on the HPA axis. This response helps regulate stress and appetite during food deprivation.

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Area of Science:

  • Neuroendocrinology
  • Stress Physiology
  • Molecular Biology

Background:

  • Repeated stress reduces glucocorticoid receptor (GR) mRNA in the hypothalamus and hippocampus, impairing negative feedback and causing HPA axis hyperactivity.
  • Starvation is a stress state involving central nervous system counterregulatory responses.

Purpose of the Study:

  • To investigate the impact of 4-day starvation on the hypothalamic-pituitary-adrenocortical (HPA) axis, GR, and mineralocorticoid receptor (MR) mRNAs in specific rat brain regions and the anterior pituitary.
  • To understand the regulatory mechanisms of GR mRNA during starvation-induced stress.

Main Methods:

  • Rats were subjected to a 4-day starvation period.
  • Plasma corticosterone and ACTH levels were measured.
  • mRNA levels of GR, MR, POMC, and CRH receptors were quantified in the hippocampus, PVN, and anterior pituitary using quantitative PCR.

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Main Results:

  • Starved rats exhibited elevated plasma corticosterone but unchanged ACTH, POMC mRNA, and type-1 CRH receptor mRNA in the anterior pituitary.
  • CRH mRNA and type-1 CRH receptor mRNA decreased in the PVN.
  • GR mRNA levels remained unchanged in the hippocampus, PVN, and anterior pituitary, despite high corticosterone.
  • MR mRNA significantly decreased in the dentate gyrus and anterior pituitary.

Conclusions:

  • The preservation of GR mRNA during starvation suggests intact glucocorticoid negative feedback, contributing to decreased CRH levels in the PVN.
  • GR mRNA regulation in the central nervous system is not solely dependent on circulating glucocorticoids.
  • Further research is needed to elucidate the mechanisms governing GR mRNA regulation in the brain under stress.