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Related Experiment Videos

Cytoplasmic transfer in assisted reproduction.

J Barritt1, S Willadsen, C Brenner

  • 1Gamete and Embryo Research Laboratory, Institute for Reproductive Medicine and Science of Saint Barnabas, West Orange, New Jersey, 07052, USA. jason.barritt@embryos.net

Human Reproduction Update
|July 31, 2001
PubMed
Summary

Cytoplasmic transfer, involving the transfer of ooplasm into human oocytes, has resulted in nearly 30 healthy births globally. This assisted reproduction technique may improve oocyte function, but its full impact on early embryo physiology requires further investigation.

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Area of Science:

  • Reproductive biology
  • Assisted reproductive technology
  • Human embryology

Background:

  • Cytoplasmic transfer involves introducing ooplasm from donor oocytes or zygotes into recipient oocytes.
  • This technique is used for oocytes with probable deficiencies, particularly in patients experiencing recurrent implantation failure.
  • The potential benefits and risks of cytoplasmic transfer in assisted reproduction are still under investigation.

Purpose of the Study:

  • To review experimental cytoplasmic transfer techniques in human oocytes.
  • To explore the potential impact of cytoplasmic transfer on assisted reproduction outcomes.
  • To discuss the components transferred, including mRNAs, proteins, and mitochondria.

Main Methods:

  • Introduction of a small amount of ooplasm from donor oocytes or zygotes.

Related Experiment Videos

  • Application in human oocytes, particularly those from patients with a history of implantation failure.
  • Clinical application in several in vitro fertilization (IVF) clinics.

    • Main Results:

    • Successful pregnancies and the birth of nearly 30 healthy babies worldwide.
    • The transfer involves crucial cellular components like mRNAs, proteins, and mitochondria.
    • Pregnancies have resulted from its use in IVF clinics, indicating potential efficacy.

    Conclusions:

    • Cytoplasmic transfer has led to successful births in assisted reproduction.
    • The precise effects of transferred cytoplasm on early embryo physiology remain uncertain.
    • Further research is needed to fully understand the impact and optimize techniques for assisted reproduction.