Related Experiment Video
Updated: Jul 23, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Characterization of domains in C/EBPalpha that mediate its constitutive and cAMP-inducible activities
H L Wilson1, P J McFie, W J Roesler
1Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, S7N 5E5, Saskatchewan, Canada.
Abstract:
Structure/function analysis of CCAAT/enhancer binding proteins (C/EBP) alpha and beta have shown that they possess both constitutive and cAMP inducible activities. Three regions conserved between C/EBPalpha and beta were identified which lie within the cAMP inducible domains of each protein. Deletion analysis of these conserved regions within C/EBPalpha show that conserved region 2 plays a particularly critical role in mediating the PKA inducible activity of the protein, however, the constitutive activity of conserved region 2 depends on promoter context. This data supports previous findings that constitutive and cAMP responsiveness are mediated by domains of the protein that do not directly overlap, suggesting that they occur through distinct mechanisms.
Related Concept Videos
RNA Polymerase II Accessory Proteins
Cell Specific Gene Expression
Master Transcription Regulators
Assembly of Signaling Complexes
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Constitutive and Regulated Gene Expression

