Related Experiment Video
Updated: Oct 5, 2026
Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
[Augmented effect of Composite Radix Salviae Miltiorrhizae Injection on radiosensitivity of leukemia cells]
Objective:
To explore the effect of Composite Radix Salviae Miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells.
Methods:
Semisolid agar culture and flow cytometry assay were performed for studying the change in radiosensitivity of HL60 cell line and fresh human leukemia cells after exposing to CSMI.
Results:
The D0(the inverse of the slope of the survival curve) and SF2(survival fraction at 2 Gy) of HL60 cell line were decreased from 1.53, 0.34 to 0.93, 0.12, respectively and apoptosis rates after radiation were raised significantly by CSMI. Furthermore, the concentration of CSMI and the time of mixed culture with CSMI before irradiation had positive relation to the effects mentioned above. Compared with control group, CSMI could increase the radiation-induced apoptosis of fresh leukemia cells (5.89 +/- 2.91% vs 12.05 +/- 3.06%).
Conclusion:
CSMI could obviously enhance the radiosensitivity of leukemia cells.