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Related Experiment Videos

Human antibody expression in transgenic mice.

M Brüggemann1

  • 1Laboratory of Developmental Immunology, The Babraham Institute, Cambridge, UK. marianne.bruggemann@bbsrc.ac.uk

Archivum Immunologiae Et Therapiae Experimentalis
|August 2, 2001
PubMed
Summary

Transgenic mice carrying human immunoglobulin genes can produce diverse human antibody repertoires. This technology enables the development of high-affinity human monoclonal antibodies for therapeutic applications.

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Area of Science:

  • Immunology
  • Genetics
  • Biotechnology

Background:

  • Human antibody repertoires are crucial for therapeutic applications.
  • Generating diverse human antibody repertoires in mice presents technical challenges.

Purpose of the Study:

  • To establish a method for creating human antibody repertoires in transgenic mice.
  • To produce high-affinity human monoclonal antibodies for therapeutic use.

Main Methods:

  • Introduction of human immunoglobulin heavy and light chain genes into mouse germline.
  • Utilizing yeast artificial chromosomes and chromosome fragments for transgene constructs.
  • Employing pronuclear DNA injection and embryonic stem cell transfection for integration.
  • Achieving faithful rearrangement and V(D)J recombination of human DNA segments.

Main Results:

  • Successful generation of transgenic mice with human immunoglobulin genes.
  • Demonstrated faithful rearrangement and extensive V(D)J combinations of human DNA.
  • Production of specific human monoclonal antibodies with high affinity.

Conclusions:

  • Transgenic mice with human immunoglobulin genes can effectively generate diverse antibody repertoires.
  • This approach is a viable strategy for producing human monoclonal antibodies for therapeutic applications.

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