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Modification of collagen film by certain chemical agents

E Lesiak-Cyganowska¹, D Sladowski, J Komender

¹Department of Transplantology and Central Tissue Bank, Center of Biostructure Research, Warsaw Medical University, Poland. elesiak@ib.amwaw.edu.pl

Archivum Immunologiae Et Therapiae Experimentalis

|August 2, 2001

Abstract:

Morphological changes and the content of free carboxyl groups in bovine collagen (type I) film under the influence of trypsin, hydrochloric acid (HCl) and ethylenediaminetetraacetic acid (EDTA) were studied. Incubation with trypsin and HCl was found to cause some delamination of the film and the appearance of some low-density spots. Incubation with EDTA did not cause any morphological changes. A high concentration of free carboxyl groups (10-fold higher than in control) was seen after incubation with trypsin.

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