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Published on: April 14, 2010
Differential effects of IL-2 and IL-15 on expression of IL-2 receptor alpha
A Alileche1, C K Goldman, T A Waldmann
1Metabolism Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA.
Abstract:
IL-2 and IL-15 have overlapping functions since they share the IL-2Rbetagamma receptor complex. However, each cytokine has a private alpha receptor namely IL-2Ralpha for IL-2 and IL-15Ralpha for IL-15. As a consequence the effects of the two cytokines may differ. We describe the differential effects of the two cytokines regarding the induction of cell surface expression of the IL-2Ralpha subunit on YT-l cells. Both cytokines induced transcription of the IL-2Ralpha gene. Furthermore translation of IL-2Ralpha leading to intracellular expression of the receptor was observed following either IL-2 or IL-15 addition. However, only IL-15 was associated with the induction of cell surface expression of IL-2Ralpha. With IL-2 there appears to be an impediment to the translocation of IL-2Ralpha to the cell membrane. Since surface expression of IL-2Ralpha is a key element in the formation of the high affinity IL-2 receptor, translocation of IL-2Ralpha to the membrane represents another level of control of the immune response in addition to regulation of IL-2Ralpha transcription and translation.

