Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacteriophage lambda DNA packaging: DNA site requirements for termination and processivity.

D Cue1, M Feiss

  • 1Department of Microbiology, University of Iowa, Iowa City, IA, USA.

Journal of Molecular Biology
|August 2, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic dissection of the Streptococcus pyogenes M1 protein: regions involved in fibronectin binding and intracellular invasion.

Microbial pathogenesis·2001
Same author

The functional asymmetry of cosN, the nicking site for bacteriophage lambda DNA packaging, is dependent on the terminase binding site, cosB.

Biochemistry·2001
Same author

Defining cosQ, the site required for termination of bacteriophage lambda DNA packaging.

Genetics·2001
Same author

Endonuclease and helicase activities of bacteriophage lambda terminase: changing nearby residue 515 restores activity to the gpA K497D mutant enzyme.

Virology·2000
Same author

ATPase center of bacteriophage lambda terminase involved in post-cleavage stages of DNA packaging: identification of ATP-interactive amino acids.

Journal of molecular biology·2000
Same author

High frequency invasion of mammalian cells by beta hemolytic streptococci.

Sub-cellular biochemistry·2000
Same journal

Tesorai Search: cloud-based database search engine boosts identifications for mass spectrometry proteomics with a pretrained peptide-spectrum deep-learning model.

Journal of molecular biology·2026
Same journal

Characterization of diverse functions of NRF1 nuclear localization sequence.

Journal of molecular biology·2026
Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026
Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026
Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026
Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026
See all related articles

Bacteriophage lambda DNA packaging requires specific DNA segments for efficient termination and processivity. An initiation-defective cosB segment still supports processivity, suggesting less stringent interactions during this phase.

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Bacteriophage lambda DNA is packaged into viral shells using intracellular DNA multimers.
  • The cos site, a 200 bp DNA segment, contains essential sequences for DNA packaging.

Purpose of the Study:

  • To investigate the specific DNA segments required for efficient termination and processivity during bacteriophage lambda DNA packaging.
  • To analyze the role of the cosB segment and its interactions with terminase during initiation and processivity.

Main Methods:

  • Analysis of DNA packaging efficiency using various cos site mutants.
  • Characterization of terminase-cosB interactions in initiation and processivity.

Main Results:

  • Efficient DNA packaging termination requires the I2 segment, cosQ, and cosN.

Related Experiment Videos

  • Efficient processivity requires cosB, cosQ, and cosN.
  • An initiation-defective cosB mutant still supported efficient processivity, indicating less stringent terminase-cosB interactions for termination.

    • Conclusions:

    • The I2 segment is crucial for efficient DNA packaging termination in bacteriophage lambda.
    • The cosB segment's interactions with terminase are less stringent for processivity than for initiation.
    • This finding re-interprets previous observations regarding phage 21 cosB and lambda packaging machinery.