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ROSE elements occur in disparate rhizobia and are functionally interchangeable between species.

A Nocker1, N P Krstulovic, X Perret

  • 1Institut für Mikrobiologie, Eidgenössische Technische Hochschule, ETH-Zentrum, Schmelzbergstrasse 7, 8092 Zürich, Switzerland.

Archives of Microbiology
|August 2, 2001
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Summary
This summary is machine-generated.

Heat shock gene expression in bacteria is regulated by ROSE elements, found across multiple rhizobia species. These elements control small heat shock protein (sHsp) production, showing conserved function in heat response.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The regulation of heat shock gene expression is crucial for bacterial survival under stress.
  • Small heat shock proteins (sHsps) play a vital role in cellular protection during thermal stress.
  • The ROSE (repression of heat shock gene expression) element controls gene expression in Bradyrhizobium japonicum.

Purpose of the Study:

  • To investigate the presence and function of ROSE elements in various rhizobia species.
  • To determine if the regulatory mechanism conferred by ROSE elements is conserved across different rhizobia.
  • To provide genetic evidence for multiple sHsps in these organisms.

Main Methods:

  • Sequence alignment of ROSE elements from different rhizobia.
  • Primer-extension analysis to study transcription of ROSE-associated operons.
  • Construction and analysis of translational ROSE-hsp'-'lacZ fusions in B. japonicum.

Main Results:

  • ROSE elements are present in Bradyrhizobium sp. (Parasponia), Rhizobium sp. strain NGR234, and Mesorhizobium loti, in addition to B. japonicum.
  • A conserved region within the ROSE element suggests functional importance.
  • Transcription of ROSE-controlled operons is repressed at low temperatures and induced upon temperature upshift.
  • Heterologous ROSE elements from other rhizobia species confer heat-responsiveness when integrated into B. japonicum.

Conclusions:

  • The ROSE regulatory element and its function in controlling heat shock gene expression are conserved among various rhizobia species.
  • This conserved regulatory principle highlights a common adaptation strategy in rhizobia.
  • The findings support the presence of multiple sHsps in these bacterial groups.