In vivo thrombolysis. An experimental model in rhesus monkeys

An experimental model of diffuse thromboembolism in the intrarenal circulation of the rhesus monkey was produced by intra-arterial injection of homogenised, homologous [125-I]fibrin. A sequential study of thromolysis was carried out using the conventional method of determining the thromboembolic index in the arteries of both kidneys together with radioautography and counting of radioactivity in the renal homogenate. Quantitative estimation of radioactivity in the renal homogenate, as well as radioautography of kidney sections provided more accurate and sensititve indices of the thrombolytic process than the conventional thrombembolic index. Studies on blood coagulation and fibrinolysis revealed only mild changes corresponding to the dynamics of thrombolysis in vivo. It was further noted that the thrombolytic mechanism in rhesus monkeys is very active and can clear thromboemboli from the intrarenal arteries within 8 days of their induction