Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Image metrics in the statistical analysis of DNA microarray data.

C S Brown1, P C Goodwin, P K Sorger

  • 1Biotechnology Group, Applied Precision, Inc., 1040 12th Avenue Northwest, Issaquah, WA 98027, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 2, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis insights to support the use of wastewater and environmental surveillance data for infectious diseases and pandemic preparedness.

Epidemics·2025
Same author

A case of penicillin-resistant group B Streptococcus isolated from a patient in the UK.

The Journal of antimicrobial chemotherapy·2024
Same author

Determining people's attitudes and motivation towards their health in patients with low back pain using the Health Styles questionnaire. A test of feasibility and validity.

Musculoskeletal science & practice·2024
Same author

Detection, survival, and persistence of Staphylococcus capitis NRCS-A in neonatal units in England.

The Journal of hospital infection·2023
Same author

A carcinoid tumor of the middle ear masquerading as a glomus tympanicum presenting with temporal lobe hemorrhage in a 70-year-old woman: Case report and review of the literature.

Neuro-Chirurgie·2022
Same author

First experience of implementing Candida auris real-time PCR for surveillance in the UK: detection of multiple introductions with two international clades and improved patient outcomes.

The Journal of hospital infection·2022

Statistical analysis of DNA microarrays reveals significant uncertainty in gene expression quantification due to image artifacts. Pixel-level analysis and weighting schemes can improve data quality for more accurate gene expression profiling.

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • DNA microarrays are crucial for determining cellular expression profiles.
  • Spotted microarrays involve hybridizing fluorescently labeled cDNA to DNA targets on slides.
  • Accurate quantification of gene expression is essential for biological research.

Purpose of the Study:

  • To statistically analyze DNA microarray images and identify sources of uncertainty.
  • To develop methods for estimating and improving the accuracy of gene expression ratios.
  • To propose a probabilistic framework for DNA microarray data analysis.

Main Methods:

  • Statistical analysis of microarray images at the pixel level.
  • Quantification of fluorescent DNA binding and assessment of intensity fluctuations.

Related Experiment Videos

  • Development and application of simple weighting schemes based on error estimates.

    • Main Results:

    • Quantitating DNA bound to microarrays is subject to considerable uncertainty.
    • Sources of error include intensity fluctuations, nonadditive background, and fabrication artifacts.
    • Pixel-by-pixel analysis effectively estimates error sources and improves gene expression ratio accuracy.

    Conclusions:

    • Image-based error metrics are vital for assessing DNA microarray data quality.
    • Weighting schemes significantly enhance data quality in multiexperiment databases.
    • An explicitly probabilistic approach incorporating error estimates is proposed for microarray data analysis.