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Effect of hyaluronan on MMP expression and activation
N Isnard1, J M Legeais, G Renard
1Laboratoire de Recherche en Ophtalmologie, Esc B3 6ème étage, Hôpital Hôtel Dieu, 1 place du Parvis Notre-Dame, Paris, 75004, France. n.isnard@extracellular.matrix.com
Cell Biology International
|August 3, 2001
Summary
Hyaluronan significantly boosts matrix metalloproteinase (MMP) expression and activity in human skin fibroblasts and corneal keratocytes. This suggests hyaluronan plays a key role in regulating MMPs during tissue remodelling.
Area of Science:
- Biochemistry and Molecular Biology
- Cell Biology
- Dermatology and Ophthalmology
Background:
- Matrix metalloproteinases (MMPs) are critical enzymes in tissue remodeling, involved in both physiological and pathological processes.
- Hyaluronan, a key matrix component, also participates in similar cellular functions.
- Cytokines and growth factors regulate the biosynthesis and activity of both hyaluronan and MMPs.
Purpose of the Study:
- To investigate the relationship between hyaluronan and MMPs.
- To determine the effect of hyaluronan on MMP expression (biosynthesis and activation) in human skin fibroblasts and corneal keratocytes.
Main Methods:
- Utilized explant and cell cultures of human skin fibroblasts and corneal keratocytes.
- Measured elastase-type endopeptidase activity using a synthetic substrate (N-Suc(ala)3pNA).
- Characterized MMPs via zymography.
Main Results:
- Hyaluronan addition stimulated membrane-bound elastase-type endopeptidase activity in a dose-dependent manner in both cell types.
- Observed increased MMP expression and activation of latent MMPs in the presence of 1 mg/ml hyaluronan.
Conclusions:
- Hyaluronan actively influences MMP expression and activation in fibroblasts and keratocytes.
- These findings highlight a significant role for hyaluronan in modulating MMP activity, impacting tissue remodeling processes.