Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasma cholesterol ester formation in the neonatal lamb.

R C Noble, M L Crouchman, J H Moore

    Biology of the Neonate
    |January 1, 1975
    PubMed
    Summary

    Newborn lamb plasma shows immediate lecithin-cholesterol-acyltransferase (LCAT) activity, which increases within 24 hours. However, LCAT efficiency is severely inhibited at birth, despite rising lipid substrates.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Uncovering Dental Caries Heterogeneity in NHANES Using Machine Learning.

    Journal of dental research·2025
    Same author

    Continued Fever, or a So-Called Ridge Fever, in Upper South Carolina.

    Atlanta journal-record of medicine·2022
    Same author

    Painless Vaccination.

    Atlanta medical and surgical journal (1884)·2022
    Same author

    Metabolomics Insights in Early Childhood Caries.

    Journal of dental research·2021
    Same author

    Electrophysiology-based stratification of pancreatic tumorigenicity by label-free single-cell impedance cytometry.

    Analytica chimica acta·2020
    Same author

    How to increase our belief in discovered statistical interactions via large-scale association studies?

    Human genetics·2019

    Area of Science:

    • Biochemistry
    • Neonatal Physiology
    • Lipid Metabolism

    Background:

    • Lecithin-cholesterol-acyltransferase (LCAT) is crucial for cholesterol esterification in plasma.
    • Understanding LCAT activity in newborns is vital for assessing lipid metabolism and cardiovascular health.
    • Neonatal lipid profiles and enzyme activities can differ significantly from adults.

    Purpose of the Study:

    • To investigate the activity and efficiency of the LCAT enzyme in newborn lamb plasma.
    • To determine the temporal changes in LCAT activity during the first 24 hours postpartum.
    • To correlate LCAT activity with changes in plasma lipid substrates.

    Main Methods:

    • Enzyme activity assays were performed on plasma samples from newborn lambs.
    • LCAT activity was measured at birth and at 24-hour intervals.
    • Plasma lipid concentrations, including cholesterol and triglycerides, were quantified.

    Main Results:

    • LCAT activity was detectable in newborn lamb plasma immediately after birth.
    • A significant increase in LCAT system activity was observed within the first 24 hours postpartum.
    • This increase in activity correlated with elevated plasma concentrations of lipid substrates.
    • Evidence indicated severe inhibition of LCAT mechanism efficiency in the immediate postnatal period.

    Conclusions:

    • Newborn lambs possess LCAT activity at birth, which undergoes a marked increase within 24 hours.
    • Despite increased substrate availability, the efficiency of the LCAT system is compromised in the immediate neonatal period.
    • These findings highlight a unique aspect of lipid metabolism regulation in neonatal lambs, potentially impacting cholesterol homeostasis.

    Related Experiment Videos