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Related Experiment Videos

G-overhang dynamics at Tetrahymena telomeres.

N K Jacob1, R Skopp, C M Price

  • 1Department of Molecular Genetics, Biochemistry and Microbiology, School of Medicine, University of Cincinnati, ML0524, 231 Albert Sabin Way, Cincinnati, OH 45267, USA.

The EMBO Journal
|August 3, 2001
PubMed
Summary

The DNA G-strand overhang at Tetrahymena telomeres is tightly regulated, typically ending in 5'-TTGGGGT. Its length is maintained during the cell cycle but changes upon cell cycle exit, suggesting altered DNA processing.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Telomeres protect chromosome ends from degradation and fusion.
  • The G-rich strand overhang at telomeres plays a crucial role in telomere maintenance and replication.
  • Understanding telomere structure is vital for comprehending genome stability and aging.

Purpose of the Study:

  • To precisely measure the length of the G-strand overhang at Tetrahymena thermophila telomeres.
  • To investigate the regulation of telomere overhang structure during the cell cycle.
  • To explore the presence and implications of overhangs in rDNA molecules.

Main Methods:

  • Ligation-mediated primer extension protocol for accurate overhang length measurement.
  • Analysis of telomere structure across different cell cycle stages.

Related Experiment Videos

  • Cloning of rDNA molecules dependent on telomeric overhangs.

    • Main Results:

    • Telomere G-strand overhang length and terminal nucleotide are tightly regulated, predominantly terminating in 5 -TTGGGGT.
    • >80% of overhangs are 14-15 or 20-21 nucleotides long.
    • Overhang length is stable during the cell cycle but changes upon cell cycle exit, indicating altered DNA processing or end protection.

    Conclusions:

    • Telomere G-strand overhang generation is a highly regulated process involving multiple DNA-processing events.
    • rDNA molecules possess overhangs at both telomeres, enabling cloning strategies.
    • A significant fraction of leading strand telomeres exhibit overhangs of at least 5 nucleotides.