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Updated: Oct 5, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Effects of phenytoin on structural aberration of human sperm chromosomes in vitro]
1Department of Psychiatry, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016 P.R.China. MHQ99666@sina.com
Objective:
To detect the mutagenic effects of phenytoin on human sperm chromosomes.
Methods:
The mutagenic effects of phenytoin at 10, 20 and 40 microgram/ml were tested by an in vitro testing system of human sperm chromosomes, with leomycin A5 as a positive control and mutagen-free solution as the blank control.
Results:
The frequencies of sperm with structural chromosomal aberrations and breakages of sperm chromosomes in the groups of 10 microgram/ml, 20 microgram/ml and 40 ug/ml of phenytoin were all higher than those in the blank control, but only in the group of 40 ug/ml, the elevated frequencies were statistically significant (P<0.005, P<0.05).
Conclusion:
Phenytoin has probably mutagenic potential effect on human sperm cells.
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