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Related Experiment Videos

Tissue transglutaminase expression in breast carcinomas.

M Y Grigoriev1, E N Suspitsin, A V Togo

  • 1N.N. Petrov Institute of Oncology, St. Petersburg, Russia.

Journal of Experimental & Clinical Cancer Research : CR
|August 4, 2001
PubMed
Summary

Tissue transglutaminase (tTG) RNA was detected in 44% of breast cancers, correlating with high apoptosis. However, tTG protein was rarely found in tumor cells, suggesting complex expression patterns in breast carcinoma.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Tissue transglutaminase (tTG) is implicated in cellular processes like apoptosis and adhesiveness.
  • Dysregulation of tTG expression is linked to diseases including cancer and AIDS.
  • Understanding tTG's role in breast cancer is crucial for disease mechanism insights.

Purpose of the Study:

  • To investigate the expression patterns of tTG in breast carcinoma.
  • To correlate tTG expression with apoptotic activity and clinical parameters.
  • To assess the relationship between tTG RNA and protein levels in breast tumors.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) to detect tTG RNA.
  • Immunohistochemistry to evaluate tTG protein expression in tumor cells and stroma.

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  • Statistical analysis to correlate findings with apoptotic index and clinical data.

    • Main Results:

    • tTG RNA was detected in 44% of breast cancer samples.
    • tTG message showed a significant association with a high apoptotic index (p=0.03).
    • tTG protein was observed in tumor cells in only 15% of cases, with stromal staining in about 50%.

    Conclusions:

    • A significant discordance exists between tTG RNA and protein expression in breast carcinomas.
    • Immunohistochemistry sensitivity may limit detection of functionally relevant tTG protein.
    • tTG RNA expression might reflect transcriptional activation of other pathways rather than direct protein accumulation.