Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glycol methacrylate: an alternative method for embedding subcutaneous implants.

J E Gomes-Filho1, B P Gomes, A A Zaia

  • 1Dental Faculty of Piracicaba, Brazil.

Journal of Endodontics
|August 4, 2001
PubMed
Summary

Glycol methacrylate (GMA) embedding improves visualization of inflammatory cells in rat tissue samples. This technique enhances biocompatibility assessment of endodontic sealers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellular and molecular immunomodulatory potential of red wine polyphenols in apical periodontitis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2026
Same author

Influence of dealcoholized red wine on the healing of bone with induced apical periodontitis in rats.

Brazilian journal of biology = Revista brasleira de biologia·2026
Same author

Influence of photodynamic therapy on bond strength and adhesive interface morphology of MTA based root canal sealer to different thirds of intraradicular dentin.

Photodiagnosis and photodynamic therapy·2020
Same author

Reduced bone resorption and inflammation in apical periodontitis evoked by dietary supplementation with probiotics in rats.

International endodontic journal·2020
Same author

Efficacy of reciprocating and ultrasonic activation of 6% sodium hypochlorite in the reduction of microbial content and virulence factors in teeth with primary endodontic infection.

International endodontic journal·2019
Same author

Systemic administration of probiotics reduces the severity of apical periodontitis.

International endodontic journal·2019

Area of Science:

  • Biomaterials Science
  • Histology
  • Endodontics

Background:

  • Evaluating endodontic sealer biocompatibility requires precise histological analysis.
  • Traditional embedding methods may limit cellular detail.

Purpose of the Study:

  • To investigate glycol methacrylate (GMA) as an embedding material for rat subcutaneous tissue.
  • To assess the efficacy of GMA in preserving cellular detail for inflammatory cell analysis.

Main Methods:

  • Rat subcutaneous tissue with endodontic sealer implants was fixed and dehydrated.
  • Specimens were infiltrated and embedded using glycol methacrylate (GMA).
  • Blocks were sectioned into 3.0 micrometer slices and stained with hematoxylin-eosin.

Main Results:

Related Experiment Videos

  • GMA embedding provided excellent cell definition and staining quality.
  • Quantitative analysis of infiltrated cells and identification of inflammatory cell types were possible.
  • Distinction between acute and chronic inflammatory cells was clear.

Conclusions:

  • Glycol methacrylate (GMA) embedding is an easy, reproducible technique.
  • It offers superior tissue cell definition for evaluating inflammatory responses.
  • GMA is an excellent alternative for assessing endodontic sealer biocompatibility.