Related Experiment Videos
Matrix metalloproteinase expression after human cardioembolic stroke: temporal profile and relation to neurological
J Montaner1, J Alvarez-Sabín, C Molina
1Cerebrovascular Unit, Hemostasia Research Unit, Vall d'Hebron Hospital, Barcelona, Spain. alsa@hg.vhebron.es
Background And Purpose:
Uncontrolled expression of matrix metalloproteinases (MMPs) can result in tissue injury and inflammation. In animal models of cerebral ischemia, the expression of MMP-2 and MMP-9 was significantly increased. However, their role in human stroke in vivo remains unknown. Therefore, we sought to determine the temporal profile of MMP expression in patients with acute ischemic stroke and to investigate its relationship to stroke severity, location of arterial occlusion, and total infarct volume.
Methods:
Serial MMP-2 and MMP-9 determinations were made in 39 patients with cardioembolic strokes that involved the middle cerebral artery territory by means of enzyme-linked immunosorbent assay. Blood samples, transcranial Doppler recordings, and National Institutes of Health Stroke Scale (NIHSS) scores were obtained at baseline and at 12, 24, and 48 hours after stroke onset. Infarct volume was measured with CT scanning at 48 hours.
Results:
No correlation was found between MMP-2 and NIHSS score at any time point, although a close relation appeared between mean MMP-9 and final NIHSS score (r=0.486, P=0.002). MMP-9 value was the only factor associated with the final NIHSS score in the multiple logistic regression model (OR 4.54, 95% CI 1.5 to 13.75). A cut-point of MMP-9 142.18 ng/mL had a positive predictive value of 94.4% to assess a patient's NIHSS (<8 or >/=8) by the end of the study. Final MMP-2 and MMP-9 levels were significantly lower when recanalization occurred (528+/-144.3 versus 681.4+/-239.2 ng/mL, P=0.031 for MMP-2; 110.2+/-100.9 versus 244.8+/-130 ng/mL, P=0.004 for MMP-9). A positive correlation was found between mean MMP-9 and infarct volume (r=0.385, P=0.022).
Conclusions:
MMPs are involved in the acute phase of human ischemic stroke. MMP-9 levels are associated with neurological deficit, middle cerebral artery occlusion, and infarct volume.
Insights
Matrix metalloproteinase-9 (MMP-9) levels correlate with stroke severity and infarct volume in acute ischemic stroke patients. MMP-9 is a key factor in human stroke, impacting neurological deficits and outcomes.
Area of Science:
- Biochemistry
- Neurology
- Cardiovascular Research
Background:
- Uncontrolled matrix metalloproteinases (MMPs) contribute to tissue injury and inflammation.
- MMP-2 and MMP-9 expression increases in animal models of cerebral ischemia.
- The role of MMPs in human ischemic stroke in vivo is not well understood.
Purpose of the Study:
- To determine the temporal profile of MMP expression in acute ischemic stroke patients.
- To investigate the relationship between MMP levels, stroke severity, arterial occlusion location, and infarct volume.
Main Methods:
- Serial MMP-2 and MMP-9 measurements using ELISA in 39 patients with cardioembolic middle cerebral artery strokes.
- Blood samples, transcranial Doppler, and NIHSS scores collected at baseline and 48 hours post-stroke.
- Infarct volume assessed by CT scanning at 48 hours.
Main Results:
- MMP-9 levels strongly correlated with final NIHSS scores (r=0.486, P=0.002) and were the sole predictor in logistic regression (OR 4.54).
- A MMP-9 cut-point of 142.18 ng/mL accurately predicted NIHSS scores.
- MMP-9 levels were positively correlated with infarct volume (r=0.385, P=0.022) and lower with recanalization.
Conclusions:
- MMPs play a role in the acute phase of human ischemic stroke.
- MMP-9 levels are significantly associated with neurological deficit severity.
- MMP-9 is linked to middle cerebral artery occlusion and infarct volume in stroke patients.