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Estimating biomass yield coefficients for autotrophic ammonia and nitrite oxidation from batch respirograms.

K Chandran1, B F Smets

  • 1Environmental Engineering Program, University of Connecticut, Storrs 06269-2037, USA.

Water Research
|August 7, 2001
PubMed
Summary
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Accurate biomass yield coefficients are crucial for understanding autotrophic ammonia oxidation. Using incorrect calculations for heterotrophic processes leads to significant overestimations in autotrophic yield and growth rates.

Area of Science:

  • Environmental microbiology
  • Biochemical engineering
  • Stoichiometry

Background:

  • Batch respirometry is vital for kinetic characterization of biological processes.
  • Biomass yield coefficient links biomass synthesis, substrate consumption, and oxygen uptake.
  • Accurate yield coefficients are essential for stoichiometric modeling.

Purpose of the Study:

  • Derive accurate biomass yield coefficients for autotrophic ammonia and nitrite oxidation.
  • Compare conventional heterotrophic yield calculations with autotrophic requirements.
  • Highlight the impact of erroneous yield estimates on kinetic parameter determination.

Main Methods:

  • Developed expressions for autotrophic biomass yield coefficients.
  • Utilized a mechanistically based electron balance equation.

Related Experiment Videos

  • Applied batch respirometry data for kinetic analysis.
  • Main Results:

    • Conventional heterotrophic yield expressions result in erroneous autotrophic yield estimates.
    • Autotrophic yield coefficients were overestimated by 27–36%.
    • Overestimated yield values led to 30–40% overestimates in maximum specific growth rate.

    Conclusions:

    • Employing the correct expression for autotrophic biomass yield is essential.
    • Inaccurate yield estimation significantly impacts subsequent kinetic parameter calculations.
    • Correct stoichiometric modeling is critical for reliable biological process characterization.