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[The effects of dexamethasone on biological characteristics of bone marrow stromal cells]
1Department of Orthopedics and Traumatology, Nanfang Hospital, First Military Medical University, Guangzhou Guangdong, P. R. China 510515.
Objective:
To investigate the effects of dexamethasone on the proliferation and differentiation of bone marrow stromal cells(MSC).
Methods:
MSC were isolated and cultured in vitro. After treatment with different concentrations of dexamethasone (0, 10(-10), 10(-9), 10(-8), 10(-7) and 10(-6) mol/L), the proliferation and alkaline phosphatase (ALP) activity of MSC were measured to evaluate the effect of dexamethasone on the biological characteristics of MSC.
Results:
Dexamethasone inhibited cell proliferation. With the increase of concentration of dexamethasone, the effect was enhanced, which was more significant when the concentration of dexamethasone was over 10(-8) mol/L. At the same time, dexamethasone promoted the activity of ALP. This effect was enhanced with the increase of concentration of dexamethasone, but the alteration was small when the concentration of dexamethasone was over 10(-8) mol/L. The effects increased with the time. The activity of ALP was enhanced 2 to 4 times with the dexamethasone for 6 days.
Conclusion:
Dexamethasone inhabit the proliferation of MSC, while induce them to differentiate into osteoblasts. The appropriate concentration of dexamethasone was 10(-8) mol/L.