Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two S-adenosylmethionine synthetase-encoding genes differentially expressed during adventitious root development in

A M Lindroth1, P Saarikoski, G Flygh

  • 1Department of Forest Genetics, Swedish University of Agricultural Sciences, Uppsala. lindroth@ucla.edu

Plant Molecular Biology
|August 8, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activated platelet aggregation is transiently impaired also by a reduced dose of protamine.

Scandinavian cardiovascular journal : SCJ·2019
Same author

Rhizobial Nod factors stimulate somatic embryo development in Picea abies.

Plant cell reports·2019
Same author

Basta tolerance as a selectable and screening marker for transgenic plants of Norway spruce.

Plant cell reports·2019
Same author

Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells.

Cancer letters·2019
Same author

Diet-induced obesity leads to metabolic dysregulation in offspring via endoplasmic reticulum stress in a sex-specific manner.

International journal of obesity (2005)·2017
Same author

TET-mediated hydroxymethylcytosine at the Pparγ locus is required for initiation of adipogenic differentiation.

International journal of obesity (2005)·2017

Two S-adenosylmethionine synthetase (SAMS) genes in Pinus contorta show distinct root development expression patterns. PcSAMS1 is crucial for adventitious root initiation, while PcSAMS2 is downregulated during this process.

Area of Science:

  • Plant molecular biology
  • Biochemistry
  • Gene expression analysis

Background:

  • S-adenosylmethionine synthetase (SAMS) is vital for plant growth and development.
  • Understanding SAMS gene regulation is key to manipulating plant development, particularly root formation.

Purpose of the Study:

  • To identify and characterize SAMS cDNAs in Pinus contorta.
  • To investigate the differential expression of SAMS genes during root development.
  • To explore the functional roles and evolutionary divergence of PcSAMS1 and PcSAMS2.

Main Methods:

  • cDNA cloning and sequencing.
  • Quantitative gene expression analysis (e.g., RT-qPCR).
  • Functional complementation in Saccharomyces cerevisiae.

Related Experiment Videos

Main Results:

  • Two distinct SAMS cDNAs, PcSAMS1 and PcSAMS2, were identified in Pinus contorta.
  • PcSAMS1 shows preferential root expression and specific meristematic activity during adventitious root development.
  • PcSAMS2 is expressed in both roots and shoots but downregulated during adventitious root formation, with its activity differing from transcript levels.
  • PcSAMS1 exhibits significant divergence from other plant SAMS but retains functionality, while PcSAMS2 is highly conserved among eukaryotic SAMS.

Conclusions:

  • PcSAMS1 and PcSAMS2 play distinct roles in Pinus contorta root development.
  • Post-transcriptional regulation or other uncharacterized SAMS genes likely influence overall SAMS activity.
  • PcSAMS1 represents a highly divergent, yet functional, SAMS enzyme in plants.