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Related Experiment Videos

A microfluidic device for measuring cellular membrane potential.

J Farinas1, A W Chow, H G Wada

  • 1Cell Biology & Microfluidics Groups, Caliper Technologies Corporation, Mountain View, CA 94043, USA.

Analytical Biochemistry
|August 8, 2001
PubMed
Summary
This summary is machine-generated.

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A new lab-on-a-chip system measures cellular membrane potential using fluorescent dyes. This microfluidic device offers high sensitivity for applications in high-throughput screening and functional genomics.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Cellular membrane potential is a critical indicator of cell function.
  • Accurate measurement of membrane potential is essential for various biological studies.
  • Existing methods for membrane potential measurement can be time-consuming and require significant sample volumes.

Purpose of the Study:

  • To develop a microfluidic lab-on-a-chip system for sensitive measurement of cellular membrane potential.
  • To demonstrate the utility of the system in assaying ion channel activity.
  • To enable high-throughput screening and functional genomics studies.

Main Methods:

  • A microfluidic chip was designed to sequentially access liquid samples from a microplate.
  • Cells were mixed with samples in a microchannel and incubated with potential-sensitive fluorescent dyes (DiBAC(4)(3) and Syto 62).

Related Experiment Videos

  • Cellular fluorescence responses were detected using fluorescence detection to determine membrane potential.

    • Main Results:

    • The cellular uptake of ionic fluorophores was found to be highly dependent on membrane potential.
    • A specific ratio of anionic and cationic dye fluorescence (DiBAC(4)(3)/Syto 62) correlated with membrane potential changes.
    • The system demonstrated sensitivity, with the fluorescence ratio doubling for every 33 mV change in membrane potential.
    • Ion channel activity in human T lymphocytes was successfully assayed using this microfluidic approach.

    Conclusions:

    • The developed lab-on-a-chip system provides a sensitive and efficient method for measuring cellular membrane potential.
    • The system's low cellular and reagent consumption, coupled with high data quality, makes it suitable for high-throughput applications.
    • This microfluidic approach holds significant promise for advancing high-throughput screening and functional genomics research.