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Automated reticulocyte counting using the Sysmex RAM-1.

L Peng1, H Yang, H Jiang

  • 1Department of Laboratory Medicine, First University Hospital, West China University of Medical Sciences, Chengdu, Sichuan, 610041, Peoples Republic of China. penglim@mcwcums.com

Clinical and Laboratory Haematology
|August 8, 2001
PubMed
Summary

The Sysmex RAM-1 offers precise and reproducible reticulocyte counting, showing excellent linearity and stability. This automated method enhances accuracy compared to manual techniques and flow cytometry.

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Area of Science:

  • Hematology
  • Clinical Laboratory Science
  • Automated Blood Cell Counting

Background:

  • Accurate reticulocyte counting is crucial for diagnosing and monitoring various hematological conditions.
  • Traditional manual methods can be labor-intensive and prone to inter-observer variability.
  • Advancements in automated analyzers aim to improve the efficiency and accuracy of reticulocyte enumeration.

Purpose of the Study:

  • To evaluate the performance of the Sysmex RAM-1 analyzer for reticulocyte counting.
  • To compare the Sysmex RAM-1's results with established methods like flow cytometry and manual counting.
  • To assess the precision, linearity, stability, and accuracy of the Sysmex RAM-1 for reticulocyte enumeration.

Main Methods:

  • 113 blood samples were analyzed using the Sysmex RAM-1.

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  • Results were compared against flow cytometry (FCM) and a manual counting technique.
  • Key performance metrics including precision (coefficient of variance), carry-over, stability, linearity, and method comparison (correlation coefficients) were assessed.

    • Main Results:

    • Sysmex RAM-1 demonstrated excellent precision (CV < 5%) and minimal carry-over.
    • Reticulocyte counts remained stable after 48 hours of storage at 4°C and room temperature.
    • High linearity was observed for Sysmex RAM-1 (r=0.9998) and FCM (r=0.9974) across a wide range.
    • Good correlations were found between Sysmex RAM-1 and manual counting (r=0.9760), FCM and manual counting (r=0.9623), and Sysmex RAM-1 and FCM (r=0.9527).
    • Receiver operating characteristic (ROC) curve analysis indicated a high true-positive rate (0.95) and area under the curve (0.999) for Sysmex RAM-1.

    Conclusions:

    • The Sysmex RAM-1 provides an accurate, precise, and reproducible method for reticulocyte counting.
    • It offers a reliable alternative to manual techniques and flow cytometry for routine laboratory use.
    • The analyzer simplifies the process of reticulocyte enumeration, improving laboratory efficiency and diagnostic confidence.