Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Partial complementation of embryo defective mutations: a general strategy to elucidate gene function.

B Despres1, M Delseny, M Devic

  • 1Laboratoire Génome et Développement des Plantes, UMR CNRS 5096, Université de Perpignan, 52, Avenue de Villeneuve, 66860 Perpignan-cedex, France.

The Plant Journal : for Cell and Molecular Biology
|August 8, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular and epigenetic regulations and functions of the LAFL transcriptional regulators that control seed development.

Plant reproduction·2018
Same author

Occurence of nucleoside polyphosphates in rapidly labelled RNA preparations from radish seedlings (Raphanus sativus.).

Planta·2014
Same author

Disappearance of stored polyadenylic acid and mRNA during early germination of radish (Raphanus sativus L.) embryo axes.

Planta·2014
Same author

Ribosomal RNA synthesis in imbibing radish (Raphanus sativus) embryo axes : A biochemical and cytological study.

Planta·2013
Same author

Expression of alcohol dehydrogenase in rice embryos under anoxia.

Plant molecular biology·2013
Same author

Isolation and characterization of an unusual repeated sequence from the ribosomal intergenic spacer of the crucifer Sisymbrium irio.

Plant molecular biology·2013

The EMB 506 gene is crucial for embryo development. Rescued plants show chloroplast defects, suggesting EMB 506 protein localizes to chloroplasts and impacts plant development.

Area of Science:

  • Plant Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The EMB 506 gene is essential for plant embryo development.
  • Its precise function is obscured by severe embryo-defective phenotypes in mutants.
  • Understanding EMB 506's role requires strategies to bypass early developmental lethality.

Purpose of the Study:

  • To investigate the function of the EMB 506 gene by rescuing homozygous emb 506 mutants.
  • To determine the subcellular localization of the EMB 506 protein.
  • To explore potential roles of EMB 506 beyond embryogenesis.

Main Methods:

  • Fusion of the ABI3 promoter to the EMB 506 cDNA to create a rescue transgene (ABI3::EMB 506).
  • Transformation of Arabidopsis thaliana with the ABI3::EMB 506 transgene.

Related Experiment Videos

  • Analysis of homozygous emb 506 rescued plants, including phenotypic characterization and subcellular localization using GFP-protein fusion.

    • Main Results:

    • Homozygous emb 506 seedlings were partially rescued by the ABI3::EMB 506 transgene, allowing plantlet development.
    • Rescued plants exhibited depigmentation in rosette leaves and inflorescences, indicating chloroplast development issues.
    • GFP-fusion experiments confirmed the chloroplast localization of the EMB 506 protein.

    Conclusions:

    • The EMB 506 protein is localized to chloroplasts and plays a role in chloroplast development.
    • EMB 506 is not a ubiquitous chloroplast housekeeping protein, as its expression is not detected in all photosynthetic tissues.
    • The ABI3 promoter-driven complementation strategy is effective for studying essential embryo-defective genes and can reveal roles in vegetative stages.