Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple pathways for Cre/lox-mediated recombination in plastids.

P T Hajdukiewicz1, L Gilbertson, J M Staub

  • 1Monsanto Company, Mail Zone BB3G, 700 Chesterfield Village Parkway North, St Louis, MO 63198, USA.

The Plant Journal : for Cell and Molecular Biology
|August 8, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of ACL reconstruction tunnels on stress in the distal femur.

Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2012
Same author

Human safety and genetically modified plants: a review of antibiotic resistance markers and future transformation selection technologies.

Journal of applied microbiology·2005
Same author

Individual patient data meta-analysis of randomized controlled trials of community occupational therapy for stroke patients.

Stroke·2004
Same author

Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2003
Same author

New use of a three-dimensional pellet culture system for human intervertebral disc cells: initial characterization and potential use for tissue engineering.

Spine·2001
Same author

Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco.

The Plant journal : for cell and molecular biology·2001

The Cre/lox system efficiently removes antibiotic resistance markers from plant plastids, enabling marker-free transplastomic plants in one generation. This technology offers a valuable tool for plastid transformation and studying plastid genome dynamics.

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Plastid transformation requires selectable antibiotic resistance markers.
  • These markers are typically removed after selection, but this process can be inefficient.
  • Efficient marker removal is crucial for developing useful transplastomic crops.

Purpose of the Study:

  • To assess the feasibility of the heterologous Cre/lox recombination system for removing antibiotic resistance markers from plant plastids.
  • To evaluate the efficiency of marker excision using a visual reporter assay.
  • To investigate alternative Cre-mediated recombination pathways in plastids.

Main Methods:

  • Utilized the Cre/lox recombination system in the model plant tobacco.
  • Employed a green fluorescent protein (GFP) reporter gene assay for visual assessment of marker excision.

Related Experiment Videos

  • Combined in vivo fluorescence activation with molecular assays to confirm recombination events.

    • Main Results:

    • Demonstrated complete excision of the selectable marker from all plastid genomes early in plant development.
    • Successfully generated selectable marker-free transplastomic plants in the first seed generation.
    • Identified two alternative Cre-mediated recombination pathways leading to silent plastid genome deletions, providing insights into tRNA accumulation and trans-splicing.

    Conclusions:

    • The Cre/lox system is a highly efficient tool for generating marker-free transplastomic plants.
    • This system has significant potential applications in advancing plastid transformation technology.
    • The study revealed novel insights into plastid genome recombination mechanisms and their consequences.