Spectrofluorimetric determination of streptomycin in dosage forms and in spiked plasma using 9,10-phenanthraquinone

A simple and highly sensitive method is proposed for the fluorimetric determination of streptomycin in dosage forms and in biological fluids. The method involves the reaction of streptomycin with 9,10-phenanthraquinone in alkaline medium to give a highly fluorescent derivative. The experimental parameters were carefully studied and incorporated into the procedures. The results obtained compared favourably with those obtained by the official methods. The concentration-fluorescence plots were rectilinear over the range 0.025-0.4 microg/ml, with minimum detectability (S/N=2) 0.006 microg/ml (4.19x10(-9) M). The proposed method was applied for the determination of streptomycin in dosage forms. The results obtained were in good agreement with those obtained by the official method. The proposed method was further applied to the determination of streptomycin in human plasma. The percentage recovery was 101.82. A proposal of the reaction pathway was presented.