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Related Experiment Videos

Strain sensitivity differences in the Hershberger assay.

K Yamasaki1, M Sawaki, M Takatsuki

  • 1Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 3-822, Ishii, Hita, 087-0061, Oita, Japan. yamasaki-kanji@hita.cerij.or.jp

Reproductive Toxicology (Elmsford, N.Y.)
|August 8, 2001
PubMed
Summary

The Hershberger assay detects androgenic properties by measuring sex organ weights. F344 rats showed less sensitivity to Flutamide-induced changes compared to SD or Wistar rats in this assay.

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Area of Science:

  • Endocrinology
  • Toxicology
  • Pharmacology

Background:

  • The Hershberger assay is a standard method for evaluating androgenic and antiandrogenic activity.
  • This assay relies on changes in accessory sex organ weights in castrated male animals.
  • Understanding strain-specific responses is crucial for assay reliability.

Purpose of the Study:

  • To investigate potential differences in strain sensitivity within the Hershberger assay.
  • To compare the responsiveness of F344, Sprague Dawley (SD), and Wistar rat strains.
  • To assess the suitability of different rat strains for detecting antiandrogenic effects.

Main Methods:

  • Castrated male rats of F344, SD, and Wistar strains were used.
  • Animals received testosterone propionate (TP) and Flutamide (FLU).

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  • Weights of accessory sex organs, including the glans penis, ventral prostate, and seminal vesicles, were measured.

    • Main Results:

    • Flutamide significantly attenuated the testosterone propionate-induced increase in glans penis weight in SD and Wistar rats, but not in F344 rats.
    • Significant differences in accessory sex organ weights were observed among the rat strains.
    • F344 rats demonstrated lower sensitivity to Flutamide-induced changes compared to SD and Wistar rats.

    Conclusions:

    • Rat strain significantly influences the outcome of the Hershberger assay.
    • F344 rats are less suitable for detecting Flutamide-induced antiandrogenic effects compared to SD and Wistar rats.
    • Strain selection is a critical factor for optimizing the sensitivity and reliability of the Hershberger assay.