The preparation and properties of factor V from bovine plasma

1. A procedure for the purification of factor V from bovine plasma with a good yield and in a high state of purity is described. The isolation procedure involves BaSO4, adsorption, chromatography of the absorbed plasma on DEAE-Sephadex A-50, activation with thrombin of the A-50 eluate, chromatography on Amberlite CG-50 and precipitation with (NH4)2SO4. Factor V is obtained with a 160% yield and 5000-fold purification. 2. Some of the physico-chemical properties of Ac-globulin have been measured. The molecular weight was determined by electrophoresis is SDS-polyacrylamide gel and found to be 30 000. The studies employing disc electrophoresis and immunoelectrophoresis suggest the existence of several active isoforms of factor V.