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Related Experiment Videos

WBC reduction in RBC concentrates by prestorage filtration: multicenter experience.

R Yomtovian1, T Gernsheimer, S F Assmann

  • 1Blood Bank-Transfusion Medicine Service, University Hospitals of Cleveland, Cleveland, Ohio 44106, USA. ryomtovian@yahoo.com

Transfusion
|August 9, 2001
PubMed
Summary
This summary is machine-generated.

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Universal leukocyte (WBC) reduction filtration in routine practice shows variable performance. While most red blood cell (RBC) units meet standards, some exceed residual WBC count guidelines, indicating room for improvement in WBC reduction efficacy.

Area of Science:

  • Transfusion Medicine
  • Hematology
  • Immunology

Background:

  • Universal leukocyte (WBC) reduction (ULR) is under consideration as a new standard in blood transfusion practices.
  • Limited data exist on the real-world performance of WBC-reduction filtration in clinical settings.
  • Evaluating WBC reduction in red blood cells (RBCs) is crucial to meet existing and proposed regulatory guidelines.

Purpose of the Study:

  • To assess and compare the performance of WBC-reduction filtration in RBC units against various regulatory thresholds.
  • To evaluate the efficacy of varied filtration practices in achieving target residual WBC counts.
  • To determine the percentage of WBC-reduced RBC units meeting FDA and European (EC) requirements for residual WBCs.

Main Methods:

  • A prospective study involving 11 sites from the Viral Activation Transfusion Study (VATS).

Related Experiment Videos

  • Red blood cell units underwent prestorage WBC-reduction filtration within 72 hours of collection.
  • Residual WBC counts were quantified using PCR in a central laboratory for 1869 WBC-reduced RBC units.

    • Main Results:

    • Filtration practices and mean residual WBC counts varied significantly across the 11 participating sites (p<0.001).
    • 0.8% of WBC-reduced RBC units exceeded the FDA requirement of <5 x 10^6 WBCs per unit.
    • 8.3% exceeded the proposed FDA requirement of <1 x 10^6 WBCs per unit, and 14.3% exceeded <5 x 10^5 WBCs per unit.

    Conclusions:

    • Residual WBC levels in WBC-reduced RBC units demonstrate variability within and between transfusion facilities.
    • WBC reduction filtration was largely successful, with over 99% meeting US and 91% meeting EC thresholds.
    • A measurable failure rate indicates that not all units consistently meet the specified residual WBC guidelines.