Study of interaction of mannan-BSA neoglycoconjugates with Concanavalin A

Neoglycoconjugates prepared by synthesis of oxidized mannans from Saccharomyces cerevisiae with bovine serum albumin were studied for interaction with Concanavalin A. The mannan-bovine serum albumin neoglycoproteins, different in degree of mannan oxidation used for synthesis and its content in conjugates were prepared. The interaction of these glycoconjugates with Concanavalin A by using precipitation method was investigated. The conjugates prepared at high weight ratio mannan: protein (4:1) involved 47-65% of saccharides and formed by precipitation with Concanavalin A aggregates with low content of protein. The obtained results showed that conjugates with lower content of mannan (up to 30%) are more efficacious for their aggregation with Concanavalin A than the conjugates with high content of mannan.