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Updated: Aug 10, 2026

Quantitation of Endothelial Cell Adhesiveness In Vitro
Published on: June 18, 2015
Adhesion development and the expression of endothelial nitric oxide synthase
D M Svinarich1, F M Zaher, L Holmdahl
1Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI 48201, USA. d.svinarich@wayne.edu
Objective:
This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.
Methods:
Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and beta-actin mRNA levels.
Results:
eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were < or = 3.12 x 10(-4), < or = 3.12 x 10(-4), 6.24 x 10(-4) and 2.5 x 10(-3) attomoles/microl, respectively. Beta-actin mRNA levels for all tissues were between 1.25 x 10(-1) and 6.25 x 10(-2) attomoles/microl.
Conclusion:
eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.
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