Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Channel-tunnels.

V Koronakis1, C Andersen, C Hughes

  • 1Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, UK. vk103@mole.bio.cam.ac.uk

Current Opinion in Structural Biology
|August 10, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The association between the experience of lay responders and response interval to medical emergencies in a rural area: an observational study.

BMC emergency medicine·2023
Same author

Fetal middle cerebral artery Doppler to time intrauterine transfusion in red-cell alloimmunization: a randomized trial.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2017
Same author

Reactions of Three Lactones with Cl, OD, and O<sub>3</sub>: Atmospheric Impact and Trends in Furan Reactivity.

The journal of physical chemistry. A·2017
Same author

Phrenic nerve block for thoracic surgery - a reply.

Anaesthesia·2017
Same author

A randomised, controlled, double-blind trial of ultrasound-guided phrenic nerve block to prevent shoulder pain after thoracic surgery.

Anaesthesia·2016
Same author

Impacts of Combustion Conditions and Photochemical Processing on the Light Absorption of Biomass Combustion Aerosol.

Environmental science & technology·2015
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

TolC proteins form a channel in bacterial outer membranes for substrate export. This channel opens via an iris-like mechanism when interacting with specific proteins.

Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • TolC and its homologues are essential outer membrane proteins in bacteria.
  • These proteins form a transperiplasmic tunnel facilitating the efflux of various substrates.
  • The structure involves an alpha-helical tunnel connected to a beta-barrel channel.

Purpose of the Study:

  • To elucidate the structural and functional mechanism of the TolC channel.
  • To understand how the periplasmic entrance of the channel is regulated.
  • To investigate the role of substrate-engaged proteins in channel opening.

Main Methods:

  • Structural analysis of TolC and related proteins.
  • Biochemical assays to study substrate binding and transport.

Related Experiment Videos

  • In vitro reconstitution of the channel and its regulatory components.

    • Main Results:

    • TolC forms a contiguous channel through the bacterial outer membrane.
    • The periplasmic entrance of the channel is typically closed.
    • An 'iris-like' mechanism opens the channel upon recruitment by cytosolic proteins.

    Conclusions:

    • TolC functions as a major bacterial efflux pump.
    • Channel gating is regulated by an iris-like mechanism at the periplasmic entrance.
    • This mechanism allows for controlled substrate export in response to cellular signals.