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Related Experiment Videos

Glucocorticoids induce basophil apoptosis.

C Yoshimura1, M Miyamasu, H Nagase

  • 1Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine, Japan.

The Journal of Allergy and Clinical Immunology
|August 10, 2001
PubMed
Summary

Glucocorticoids (GCCs) effectively induce apoptosis in basophils, key cells in allergic inflammation. This finding clarifies GCCs' anti-inflammatory mechanism in allergic disorders.

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Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Glucocorticoids (GCCs) are known to induce apoptosis in various immune cells, contributing to their anti-inflammatory effects.
  • The impact of GCCs on basophil apoptosis, crucial in allergic inflammation, was previously unclear.

Purpose of the Study:

  • To investigate the effects of glucocorticoids (GCCs) on basophil apoptosis.

Main Methods:

  • Highly purified basophils (>97% purity) were obtained using Percoll density gradient centrifugation and negative selection.
  • Cell viability and apoptosis were assessed using annexin V binding and propidium iodide exclusion assays.
  • DNA fragmentation was analyzed via flow cytometry.

Main Results:

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  • Dexamethasone (DEX), a GCC, significantly increased basophil apoptosis in a time-dependent manner.
  • Apoptosis induction by DEX was confirmed by light microscopy and DNA fragmentation assays, with a half-maximal effect at 10(-9) to 10(-8) mol/L.
  • Sex steroids did not induce apoptosis, while DEX induced it even with low-dose IL-3 present.

    • Conclusions:

    • Glucocorticoids (GCCs) demonstrate potent apoptogenic effects on basophils.
    • GCC-induced basophil apoptosis may be a key mechanism underlying the therapeutic action of these drugs in allergic conditions.