Potassium gradients in the growing hyphae of Neurospora crassa

X-ray electron probe microanalysis of a Neurospora crassa hyphal preparation revealed a significant heterogeneity of the potassium content in the hyphae. Low-potassium areas were found in a close proximity of the growing tip and in the distal part of the hyphae in the submembrane area of about 1 microm width. The intracellular potassium concentration was shown to change more than 3-5 times from apical to distal regions of the leading hyphae, the potassium contents in the apical part of the hyphae usually being minimal. We suggest that the content of intracellular potassium is determined by the volume occupied by free intracellular water, i.e., by the size of the space available for diffusion of substances, including inorganic ions.