Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multifunctional alpha-enolase: its role in diseases.

V Pancholi1

  • 1Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NewYork 10021, USA. panchov@rockvax.rockefeller.edu

Cellular and Molecular Life Sciences : CMLS
|August 11, 2001
PubMed
Summary

Enolase, a glycolytic enzyme, is a surface protein with unknown transport mechanisms. This multifunctional protein acts as a plasminogen receptor and heat-shock protein, impacting fibrinolysis and autoimmune disorders.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential expression of the enolase gene under in vivo versus in vitro growth conditions of Aeromonas hydrophila.

Microbial pathogenesis·2003
Same author

Group A streptococci bind to mucin and human pharyngeal cells through sialic acid-containing receptors.

Infection and immunity·2001
Same author

Induction of lysogenic bacteriophage and phage-associated toxin from group a streptococci during coculture with human pharyngeal cells.

Infection and immunity·2001
Same author

Antibodies to streptococcal surface enolase react with human alpha-enolase: implications in poststreptococcal sequelae.

The Journal of infectious diseases·2000
Same author

alpha-enolase, a novel strong plasmin(ogen) binding protein on the surface of pathogenic streptococci.

The Journal of biological chemistry·1998
Same author

Regulation of the phosphorylation of human pharyngeal cell proteins by group A streptococcal surface dehydrogenase: signal transduction between streptococci and pharyngeal cells.

The Journal of experimental medicine·1997

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Enolase is a key glycolytic enzyme.
  • It is a multifunctional protein found on cell surfaces.
  • Its surface transport mechanism is currently unknown.

Purpose of the Study:

  • To investigate the novel cell surface transport of enolase.
  • To explore the role of enolase as a plasminogen receptor.
  • To understand enolase's involvement in fibrinolytic and autoimmune processes.

Main Methods:

  • Analysis of enolase's surface localization.
  • Assays to determine plasminogen binding.
  • Investigation of enolase's function in various cell types.

Main Results:

Related Experiment Videos

  • Enolase is transported to the cell surface via a non-classical pathway.
  • Enolase functions as a plasminogen receptor on hematopoietic, epithelial, and endothelial cells.
  • Enolase exhibits heat-shock protein properties and binds to cytoskeletal and chromatin structures.

Conclusions:

  • Enolase's surface presence suggests a role in fibrinolysis.
  • Enolase may be implicated in autoimmune disorders.
  • Its diverse functions indicate a crucial role in transcription and pathophysiology.