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Effect of pneumoperitoneum on circulating tumor DNA
F R Lécuru1, A F Agostini, F P Coulet
1Service de Gynecologie Obstétrique, Hĵpital Boucicaut, and INSERM U 190, Université René Descartes, Paris, France.
Anticancer Research
|August 11, 2001
Summary
This study found that CO2 laparoscopy does not increase circulating tumor DNA in ovarian cancer models. Tumor biology may influence DNA release more than surgical method.
Area of Science:
- Oncology
- Surgical Oncology
- Molecular Diagnostics
Background:
- Surgery may promote the release and circulation of tumor DNA.
- Limited data exists on the specific impact of laparoscopy on circulating tumor DNA.
Purpose of the Study:
- To evaluate the effect of carbon dioxide (CO2) laparoscopy on circulating tumor DNA levels.
- To compare CO2 laparoscopy with gasless laparoscopy, midline laparotomy, and general anesthesia in terms of circulating tumor DNA release.
Main Methods:
- Ovarian cancer xenografts (IGR-OV1 and NIH:OVCAR-3) were established in nude rats.
- Surgical interventions included CO2 laparoscopy, gasless laparoscopy, midline laparotomy, and general anesthesia (control).
- Blood samples were collected post-procedure for circulating tumor DNA detection using PCR.
Main Results:
- The detection method was sensitive to 10 cells/ml of blood and specific.
- In the IGR-OV1 model, circulating tumor DNA was detected in 45-50% of cases with no significant difference between surgical groups.
- In the NIH:OVCAR-3 model, detection rates varied (22% to 64%), but overall comparison between groups was not statistically significant (p=0.2).
Conclusions:
- CO2 laparoscopy did not demonstrate a deleterious effect on circulating tumor DNA in this experimental setting.
- Tumor-specific biological characteristics may play a role in the release of circulating tumor DNA.
