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Related Experiment Videos

A novel IL-18BP ELISA shows elevated serum IL-18BP in sepsis and extensive decrease of free IL-18.

D Novick1, B Schwartsburd, R Pinkus

  • 1Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel.

Cytokine
|August 11, 2001
PubMed
Summary

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Interleukin-18 binding protein (IL-18BP) neutralizes the inflammatory cytokine IL-18. This study developed an assay to measure IL-18BPa, finding elevated levels in sepsis patients, which inhibit IL-18 activity.

Area of Science:

  • Immunology
  • Biochemistry
  • Clinical Medicine

Background:

  • Interleukin-18 (IL-18) is a pro-inflammatory cytokine implicated in Th1 immune responses.
  • IL-18 binding protein (IL-18BP) acts as a natural antagonist, inhibiting IL-18 activity by forming a high-affinity complex.
  • Understanding IL-18BP levels is crucial for evaluating IL-18-mediated inflammation, particularly in conditions like sepsis.

Purpose of the Study:

  • To develop and validate a sensitive sandwich ELISA for quantifying IL-18 binding protein a (IL-18BPa).
  • To measure serum IL-18BPa levels in healthy individuals and patients with sepsis.
  • To assess the impact of IL-18BPa on free IL-18 levels and activity in sepsis.

Main Methods:

  • Development of a sandwich ELISA for IL-18BPa with a limit of detection of 62 pg/ml.

Related Experiment Videos

  • Assessment of potential interference and cross-reactivity with related cytokines and IL-18BP isoforms.
  • Measurement of serum IL-18BPa and total IL-18 in healthy controls and septic patients using ELISA and electrochemiluminescence assays, respectively.
  • Calculation of free IL-18 levels based on the mass action law.

    • Main Results:

    • Serum IL-18BPa levels were significantly elevated in septic patients (21.9 ng/ml) compared to healthy individuals (2.15 ng/ml).
    • Total IL-18 levels were also increased in sepsis patients (1.5 ng/ml) versus healthy controls (64 pg/ml).
    • Despite elevated total IL-18 in sepsis, the majority was bound to IL-18BPa, though free IL-18 remained higher than in healthy individuals.

    Conclusions:

    • IL-18BPa effectively neutralizes circulating IL-18 in sepsis, modulating its pro-inflammatory activity.
    • The developed ELISA is a valuable tool for assessing IL-18BP levels in clinical settings.
    • Exogenous administration of IL-18BPa could be a potential therapeutic strategy to further reduce IL-18 activity in sepsis.