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Isolation, nucleotide sequence and expression of a cDNA encoding feline granulocyte colony-stimulating factor
1Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK. s.dunham@vet.gla.ac.uk
Cytokine
|August 11, 2001
Abstract:
A cDNA encoding feline granulocyte colony stimulating factor (fG-CSF) was cloned from alveolar macrophages using the reverse transcriptase-polymerase chain reaction. The cDNA is 949 bp in length and encodes a predicted mature protein of 174 amino acids. Recombinant fG-CSF was expressed as a glutathione S-transferase fusion and purified by affinity chromatography. Biological activity of the recombinant protein was demonstrated using the murine myeloblastic cell line GNFS-60, which showed an ED50 for fG-CSF of approximately 2 ng/ml.