Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Hopping, jumping and looping by restriction enzymes.

¹Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK. s.halford@bris.ac.uk

Biochemical Society Transactions

|August 11, 2001

Summary

Type II restriction enzymes like EcoRV move along DNA by hopping and jumping, not sliding. Some enzymes, like SfiI, require two DNA sites to function, enabling DNA looping analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The identification of chemical intermediates in enzyme catalysis by the rapid quench-flow technique.

Cellular and molecular life sciences : CMLS·2006

Same author

Exon 3 beta-catenin mutations are specifically associated with colorectal carcinomas in hereditary non-polyposis colorectal cancer syndrome.

Gut·2005

Same author

DNA cleavage reactions by type II restriction enzymes that require two copies of their recognition sites.

Journal of molecular biology·2001

Same author

Analysis of DNA looping interactions by type II restriction enzymes that require two copies of their recognition sites.

Journal of molecular biology·2001

Same author

SfiI endonuclease activity is strongly influenced by the non-specific sequence in the middle of its recognition site.

Nucleic acids research·2001

Same author

One- and three-dimensional pathways for proteins to reach specific DNA sites.

The EMBO journal·2000

Area of Science:

Molecular Biology
Biochemistry
Enzymology

Background:

Type II restriction endonucleases are enzymes that recognize specific DNA sequences and cleave DNA.
Many dimeric enzymes recognize palindromic sequences and can act processively, moving between sites on DNA.
Understanding enzyme translocation mechanisms is crucial for DNA manipulation and biotechnology.

Discussion:

EcoRV endonuclease primarily uses 'hopping' and 'jumping' for DNA translocation, challenging the 'sliding' model.
This non-sliding mechanism involves protein dissociation and re-association with DNA sites.
The length of DNA between sites influences the degree of enzyme processivity.

Key Insights:

EcoRV's translocation mechanism reveals novel modes of protein-DNA interaction.
SfiI, a tetrameric enzyme, requires binding to two recognition sites for activity.
SfiI's dual-site requirement facilitates DNA looping and serves as a model system for its study.

Outlook:

Further research into translocation mechanisms can refine DNA engineering tools.
SfiI provides a unique system for investigating DNA looping dynamics and protein-DNA interactions.
Understanding these enzymatic mechanisms has implications for gene editing and synthetic biology.

Related Experiment Videos