Jove
Visualize
Contact Us

Related Experiment Videos

Signalling and non-caveolar rafts.

M G Waugh1, S Minogue, J S Anderson

  • 1Centre for Molecular Cell Biology, Lower 3rd Floor, Royal Free and University College Medical School, University College London, UK.

Biochemical Society Transactions
|August 11, 2001
PubMed
Summary

Researchers identified novel lipid rafts involved in epidermal growth factor receptor signaling. They purified and cloned a key enzyme, type II phosphatidylinositol 4-kinase, revealing insights into its membrane localization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hyperparasitoids of Xenostigmus bifasciatus (Hymenoptera: Braconidae), parasitoid of Cinara spp. (Hemiptera: Aphididae), in Pinus plantations in Brazil.

Brazilian journal of biology = Revista brasleira de biologia·2026
Same author

Impairment of homeostatic structural plasticity caused by the autism and schizophrenia-associated 16p11.2 duplication.

bioRxiv : the preprint server for biology·2025
Same author

ACE gene polymorphisms (rs4340) II and DI are more responsive to the ergogenic effect of caffeine than DD on aerobic power, heart rate, and perceived exertion in a homogeneous Brazilian group of adolescent athletes.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2024
Same author

Variation of 4 MV X-ray dose rate strongly impacts biological response both in vitro and in vivo.

Scientific reports·2020
Same author

Minibeam radiation therapy at a conventional irradiator: Dose-calculation engine and first tumor-bearing animals irradiation.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2020
Same author

Minibeam radiation therapy: A micro- and nano-dosimetry Monte Carlo study.

Medical physics·2020
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Cell membranes contain specialized microdomains known as lipid rafts.
  • Lipid rafts, including caveolae, compartmentalize cellular signaling pathways.
  • The precise role of rafts in receptor-mediated phosphoinositide signaling is an emerging area of research.

Purpose of the Study:

  • To investigate the localization and function of signaling components within novel lipid raft domains.
  • To identify and characterize key enzymes involved in epidermal growth factor (EGF) receptor-dependent phosphoinositide signaling.
  • To elucidate the molecular basis for the association of type II phosphatidylinositol 4-kinase with lipid rafts.

Main Methods:

  • Density gradient centrifugation to isolate membrane fractions.

Related Experiment Videos

  • Biochemical assays to analyze phosphoinositide and receptor localization.
  • Protein purification and molecular cloning techniques.

    • Main Results:

    • Epidermal growth factor receptor, phosphatidylinositol 4-kinase, and phosphoinositides were found in caveolin-rich fractions and other novel raft types.
    • Membrane fragments containing EGF receptor and phosphoinositides could be separated from caveolae.
    • Type II phosphatidylinositol 4-kinase was successfully purified from rafts and cloned, revealing structural insights into its raft association.

    Conclusions:

    • EGF-dependent phosphoinositide signaling components localize to distinct lipid raft populations.
    • Type II phosphatidylinositol 4-kinase is a crucial raft-associated enzyme in this signaling pathway.
    • The structural characteristics of type II phosphatidylinositol 4-kinase explain its recruitment to specific membrane rafts.