Related Experiment Video
Updated: Oct 5, 2026

Fast and Accurate Exhaled Breath Ammonia Measurement
Published on: June 11, 2014
A fast chemiluminescent method for H(2)O(2) measurement in exhaled breath condensate
B Zappacosta1, S Persichilli, F Mormile
1Istituto di Chimica e Chimica Clinica and CNR Centro Chimica dei Recettori, Rome, Italy. b.zappacosta@uniserv.ccr.rm.cnr.it
Background:
Breath condensate can give useful information on volatile compounds produced at alveolar level. Actual concentration of H(2)O(2) in breath condensate is dependent on its production at alveolar level and on the efficacy of the detoxifying systems, catalase, glutathione peroxidase, etc.
Methods:
In the present paper, a simple chemiluminescent method for the determination of the H(2)O(2) collected in exhaled breath is shown and data of both smokers and nonsmokers volunteers are presented.
Results:
The chemiluminescent response is linear up to 100 micromol/l H(2)O(2). The analytical sensitivity is about 0.01 micromol/l. Most of the nonsmokers have a H(2)O(2) content lower than 0.05 micromol/l, while smokers have a content ranging from 0.1 to 0.6 micromol/l.
More Related Videos
08:44Dual Test Gas Pulmonary Diffusing Capacity Measurement During Exercise in Humans Using the Single-Breath Method
Published on: February 2, 2024
08:23Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Related Concept Videos
Flame Photometry: Lab
Flame Photometry: Overview
Gas Chromatography: Types of Detectors-II
Fast Reactions
Hess's Law
Measuring Reaction Rates