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Updated: Aug 19, 2026

Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
[Relationship between the bone marrow cell proliferation and the prognosis in childhood acute lymphoblastic leukemia]
Objective:
To study the expression of proliferative antigens in leukemic cells and the relationship between the cell proliferation activity and the prognosis.
Methods:
The labeling index (LI) of CD71, Ki-67 and PCNA in normal and leukemic cells were measured by flow cytometry.
Results:
In normal children, the LI of CD71, Ki-67 and PCNA was (32.18 +/- 16.66)%, (4.82 +/- 9.27)% and (19.69 +/- 25.11)%, respectively, while in ALL children, which was (33.66 +/- 21.52)%, (32.14 +/- 23.59)% and (47.46 +/- 30.96)%, respectively. The PCNA LI of leukemia cells was (47.46 +/- 30.96)% and (27.28 +/- 12.51)% when patients was at presentation and remitted for 3 years, respectively, and was (52.59 +/- 32.00)% and at presentation in 28 CCR children and and (26.94 +/- 14.48)% in 7 high-risk group ALL children (F = 8.877, P < 0.01), respectively.
Conclusions:
The proliferation, LI of leukemic cells was higher than that of normal cells, and reduced to normal level after treatment. The higher PCNA proliferation of untreated cells was a favourable marker for prognosis.
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