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[Effect of substance P on the short-circuit current of nasal mucosal epithelium]
1Department of Otolaryngology, First Affiliated Hospital, Shanxi Medical University, Taiyuan, 030001.
Objective:
To observe the changes of epithelial apical membrane short-circuit current (Isc) of antigen-sensitized rat nasal mucosa with the stimulation of Substances P (SP).
Methods:
The epithelial apical membrane Isc were measured with Ussing chamber technique, and and we observed the blockage to the effect of SP by neurokinin receptor antagonist Cp96345, histamine H1 receptor antagonist pyrilamine, H2 antagonist ranitidine and neurotoxin respectively.
Results:
The Isc increased significantly with the stimulation of SP. The effect of SP on Isc could be blocked significantly by pretreatment with four substances.
Conclusion:
SP releasing from nervous endings plays the role on elicit a series of pathological changes like enhanced Isc, epithelial permeabilities, etc.
