Related Experiment Video
Updated: Jan 11, 2026
Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
Published on: September 27, 2011
[Study on the methodology of potassium channel expression in Xenopus oocytes by messenger RNA from rat cochlear
Objective:
To study the properties of voltage-dependent ion channels expressed in Xenopus oocytes by mRNA from rat cochlear nucleus and to lay a methodological foundation for studying the changes of ion channels in auditory centre with sensorineural hearing loss.
Methods:
Poly (A)+ mRNAs isolated from the rat cochlear nucleus by acid guanidinum thiocyanate-phenol-chloroform (AGPC) method and Promega's new magnesphere technique were microinjected into Xenopus laevis oocytes to express functional ion channels. Voltage clamp technique was used to record the current of voltage-dependent ion channels.
Results:
A voltage-dependent ion channel was detected from oocytes injected with rat cochlear nucleus mRNA. The maximum amplitude of the outward current was 364 +/- 42 nA (n = 16). It was identified as transient outward potassium channel.
Conclusion:
The model set up by the test may play an important role in studying the physiology, pathology and pharmacology of auditory centre.
