Standardization of stinging insect extracts

The measurement of IgE Ab to insects (bee etc.) can be accomplished using RAST-discs. This prevents the non-specific wheal and flare produced when skin tests in higher concentrations of either whole body or venom are used. It will also eliminate the possibility of a serious systemic reaction by direct skin testing. The in vitro neutralization of IgE antibody by pure fractions and or components employing the RAST disc method may well be a useful tool for standardization of allergens.