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A radioimmunoassay for chemically modified grass pollen allergen
Developments in Biological Standardization
|January 1, 1975
Summary
Developing radioimmunoassays for tyrosine-adsorbed grass pollen allergens is feasible. This method allows for accurate quantification, overcoming limitations of traditional skin tests and radioallergosorbent tests for allergy diagnosis.
Area of Science:
- Allergen immunotherapy
- Immunochemistry
- Analytical biochemistry
Background:
- Chemically modified grass pollen allergens present unique physicochemical properties.
- Traditional allergy diagnostic methods like skin prick tests and radioallergosorbent tests (RAST) are unsuitable for these modified allergens.
Purpose of the Study:
- To develop and validate a reliable assay procedure for quantifying tyrosine-adsorbed grass pollen allergens.
- To establish a method for assessing the dose levels of modified allergens in preparations like Pollinex.
Main Methods:
- Development of radioimmunoassays using a labeled IgG fraction of rabbit antisera against the modified allergens.
- Evaluation of antigen concentration after solubilization to ensure assay sensitivity.
- Comparison of radioimmunoassay results with total protein assays.
Main Results:
- Radioimmunoassays proved effective for quantifying modified grass pollen allergens.
- Assay sensitivity was maintained despite solubilization procedures.
- The inhibition assay could successfully differentiate between various dose levels of Pollinex batches.
Conclusions:
- Radioimmunoassays offer a suitable alternative for assessing chemically modified grass pollen allergens.
- This approach overcomes the limitations of conventional allergy testing methods.
- The developed assay enables accurate batch consistency checks for allergen immunotherapy products.